SELECTION OF INDIGENOUS N-FIXING RHIZOBACTERIA FROM POST-TIN MINING AREAS

D Wulandari^1*, R Rosita², A F Maulana³, I Mansur⁴, Kasam^[1]

¹ Department of Environemntal Engineering, Faculty of Civil Engineering and Planning, Universitas Islam Indonesia, Yogyakarta, IndonesiaJl Kaliurang KM 14, 5 Yogyakarta, Indonesia. 55584

² SEAMEO BIOTROP, Jl Raya Tajur Bogor 16134, Indonesia

³ Department of Bioresources Technology and Veterinary, Vocational College, Universitas Gadjah Mada, Gedung SV UGM, Sekip Unit 1, Depok Sleman Yogyakarta 55281, Indonesia

⁴  Faculty of Forestry, IPB University, Dramaga Bogor 16680, Indonesia

^[1]* Corresponding author’s email: [email protected]

DOI: https://doi.org/10.20885/icsbe.vol4.art24

ABSTRACT

Tin mining is one industry that contributes to Indonesia’s economic development. However, because tin is always in high demand, this activity creates an environmental problem. Silica sand, the dominant soil in post-tin mining, is easily eroded by water and wind, resulting in soil nutrient deficiency including N. Because this condition makes it difficult for organisms to survive, rehabilitation is essential. Nitrogen (N) is an essential nutrient for plant growth. N-fixing rhizobacteria are well-known for fixing N from the atmosphere, whether through symbiosis or otherwise. The goal of this study was to isolate indigenous N-fixing rhizobacteria from a post-tin mining area. The soybean plant was used as the testing plant. Three types of N-fixing rhizobacteria were isolated from the nodule of Acacia mangium growing in a post-tin mining area: B1, B2, and B3. To minimize the nutrient content in the growth media, the plant was grown in sterilized sand. Inoculated and non-inoculated soybean were grown in a greenhouse for three months without fertilizer. There were seven replications. Among the treatments, B3 had the highest soil and pod N content, best growth performance, nodule formation, and soybean production. This finding suggests that B3 could be used for future rehabilitation in the post-tin mining area.

Keywords: post-tin mining, rehabilitation, rhizobacteria. N deficiency

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